Abstract
The nutritional requirement of Lactobacillus brevis was studied and the following result was obtained. The bacillus requires for its growth, thiamine, biotin, folic acid, nicotinic acid, pantothenic acid, uracil, cytosine and orotic acid. The bacillus may be used for the bioassay of uracil, cytosine or orotic acid. The range of assay may be 1-10 μg per ml. The inhibitory effect of thiouracil was investigated and it was confirmed that the bacillus has not the synthetic ability of aspartic acid, uredosuccinic acid or dihydroorotic acid.
