Abstract
The applicability of Farrand fluorophotometer for the determination of vitamin A was described. The vitamin A contents of liver, small intestine and blood serum were determined, and it was found that the results were similar in rats fed on standard diet. The average total vitamin A level in liver of rats was 56.6 or 9.02 μg/g and vitamin A ester was 52.9 or 8.43 μg/g. That of small intestine was 5.11 or 1.59 μg/g and that of blood serum was 29.0 μg%. The vitamin A contents of liver, small intestine and blood serum were determined one, three, six and twenty-four hours after an intravenous injection of 1 mg of vitamin A per kg of body weight to normal rats. The vitamin A content increased remarkably in the liver, and that of the liver and small intestine reached the maximum level after six hours, whereas that of blood serum decreased after the vitamin A injection.
