Abstract
It was observed that the fluorescence activity of dibenzoyl thiamine (DBT) was identical with that of thiamine in molar equivalence in the thiochrome reaction with alkaline hydrolysis. The standard curve of thiamine converted from DBT curve coincided with the calibration curve of thiamine itself by the alkaline hydrolysis method. Thiamine could therefore be used as the standard solution for determining DBT, and a part of procedure of the alkaline hydrolysis method was modified. The total amount of vitamins was determined as thiamine unit for DBT, or DBT and thiamine mixture by this modified method, and the mean value of recovery was 99.7%.
