Abstract
Studies were made in an aerobic condition on the respiratory metabolism of each component of the previously reported new synthetic medium for Bacillus thiaminolyticus. The results were as follows : It was clarified that glutamate was used as a chief energy source during the proliferation process, especially for the pellicle formation and enzyme production. Although glycerine was utilized more than glutamate as a substrate of the aerobic respiratory metabolism, the pellicle formation and enzyme production was strongly inhibited in the glycerine containing medium because of acidification of the medium. Furthermore, it was cleared that citrate was not only utilized for the stabilization of ferrous salt in the medium, but also for the spore formation.
