Abstract
The fluorescent substances in wheat seedling were separated into two fractions by the florisil adsorption treatment ; the flavins and the others. The former fraction was further fractionated by the ion exchange resin (Dowex 1×4) column chromatography, and thus, the presence of riboflavin, FMN and FAD were confirmed but no other flavin compound was detected. The latter fraction was separated to about ten fluorescent spots by paper partition chromatography. Some eluates from these spots were converted to riboflavin in the presence of cell-free extract of Aerobacter aerogenes.
