Abstract
Fractional determination of niacinamide and N'-methylniacinamide was successfully carried out by the Kato's method using Amberlite IRA-40O and IRC-50. By these procedures, cationic and anionic fluorescent impurities were completely eliminated. Then a fluorometric determination of niacinamide was made by the alkaline cyanogen bromide method, and of N'-methylniacinamide by the alkaline acetone condensation method. This method was applied to the estimation of the substances in the urine of normal, diabetic, acute hepatic and hyperthyroid patients.
