Abstract
Three kinds of fluorescent substances are found in the bile besides riboflavin. The result has shown that 0.23 and 0.46 substances are folic acid derivative, 0.80 substance is 3-hydroxyanthranilic acid. The fluorescent substances which have a great influence on the measurement of riboflavin consist of 0.23 and 0.46 substances. These fluorescent substances can be completely eliminated by oxidation with potassium permanganate.