Abstract
The conditions to activate the adsorbent (superfiltrol) and the effect of temperature of the chromato-column in assaying vitamin D by means of chromatographic method have been discussed. It has been clarified as a result that the optimal condition to separate vitamin D in the pharmaceutical preparations containing natural vitamin oil is to use the superfiltrol preheated at 130-200℃ for 1-2 hours and to keep the temperature of chromato-column at about 31℃ during the adsorption. There seem to be some unknown substances which affect the colour reaction of vitamin D by antimony trichloride in the natural vitamin oil. The authors also have succeeded in minimizing the above mentioned effect of impurities by using a specially arranged chromato-column in which the usual superfiltrol is layered on the preheated superfiltrol.
