Abstract
Previous report in our laboratory have shown that the bacterial cell fractions obtained by centrifugation of supernatant liquor from methane fermentation tank of night soil treatment was a good resources of Vitamin B_<12>. Present paper deals with the separation of vitamin B_<12> analogues in the material by the combined method of paper chromatography and paper ionophoresis. The considerable differences were found not only in the total amount but also in the proportion of the analogues by the kind of solvent, used for the extraction of the material. In the extract by KCN solution, the proportion of the analogues was cyanocobalamin 60%, factor III 20%, factor A 3%, factor B 2% and pseudo-vitamin B_<12> 5%, whereas in the aqueous extract, the total activity was only one half in the KCN extract and the proportion of cyanocobalamin was considerably low. In Na_2S_2O_5 extract, the total activity was not low but cyanocobalamin content was exceedingly small.
