Abstract
The relationship of the total riboflavin, the ratio of 3 types of riboflavin and the phosphatase activity were investigated. The results showed that the total riboflavin and the alkaline phosphatase activity were much higher in the milks of cow and goat than those of other animals. In the former, the content of free riboflavin was relatively higher than FAD and FMN. On the other hand, FAD predominated in the milks of human, pig and dog, and free riboflavin was not recognized. FAD and phosphatase of the milk were contained in the butter milk fraction.