Abstract
A new micro method for the determination of crotonobetaine contained in carnitine was developed. The method is a very simple and precise one, and not interfered by carnitine at all. The procedure is as follows : To 10 ml of a sample solution containing not more than 0.20 mg of crotonobetaine in a test tube add 1 ml of 25% H_2SO_4 and immerse the tube into water at 10℃ for 10 minutes. Add 1 ml of N/50 KMnO_4,kept at the same temperature and mix well. Determine the absorbancy of the solution at 525 mμ against water just 1 minute after the mixing of KMnO_4. Make a blank test with the same volumes of the same reagents except substituting the sample solution with water. The crotonobetaine concentration of the sample solution is obtained by comparing the decrease in color (which is obtained by substracting the absorbancy of the sample from that of the blank) with the standard curve.
