Abstract
The maximum activities of thiamine hydrochloride, thiamine propyldisulfide and S-benzoyl thiamine-monophosphate were obtained at pH 6.1 and 37-42℃. The pyrimidine or thiazole moiety had only an slight effect on the growth of the organism. However, when the moieties were used together, a significant growth stimulation was observed. Furthermore, the relation between the growth and cultivation time was investigated in each case of thiamine hydrochloride, mono- or diphosphate, or methyl disulfide and benzoyl thiamine monophosphate.