Abstract
Present paper deals with investigation on separation and determination of non-cyano type cobamides in fermentation products. An aqueous extract from feed supplement was used as a sample. Paper electrophoresis was conducted in 0.5N acetic acid and complete separation was attained for two hours. Each of separated components was eluted with potassium cyanide solution and then microbiological activity of each eluate was assayed with Ochromonas malhamensis. Results obtained showed that the aqueous extracts of feed supplement contain at least three Ochromonas malhamensis active cobamides, cyano-form (45%), hydroxoform (32%) and an unidentified form (23%). This unidentified cobamide is supposed to be protein-bound form.
