Abstract
Vitamin A-palmitate is prepared from its acetate by ester exchange reaction. The former, therefore, might be contained more or less with the latter. A simple and convenient method for the determination of acetate has been developed using infrared spectrophotometric technique. The absorption bands at 8.15 and 9.8lμ due to acetyl ester group were found to be applicable as key bands. The quantitative analysis was carried out with an 10% solution in carbon tetrachloride when acetate was contained in considerable amount. In case its content was rather small, defferential method had to be employed, with the error within 0.5%. The minimum quantity detectable by this method was 0.2-0.5%.
