Abstract
Mycelium of Aspergillus oryzae (Fugu) cultured for 48 hours under aeration in a medium containing 1% potato starch, 3% corn steep liquor and 0.2% NaNO_3 and its aqueous extract, deproteined extract, charcoal and resin treated extracts were administered to albino rats and their effects on the growth of the animal were examined in order to know in which fraction the growth factor was concentrated. However, not only any fraction, but also the mycelium itself did not exert the growth stimulating effect. Since the mycelium which has been found to be effective in previous experiments was that cultured for 24 hours, it was supposed that formation and accumulation of the effective factor may be dependent on culture conditions. Then, an examination was made on effects of mycelia obtained under such different conditions as surface culture for 48-72 hours or aerating culture for 24 hours and showed that only the mycelium used in previous experiments was effective.
