Abstract
When Propionibacterium shermanii was cultivated in the presence of hydroxocobalamin or cyanocobalamin, most of activities for Escherichia coli 215 was found to exist in the cells. In order to determine the forms and the quantities of the E. coli-active substances, paper-electrophoresis-microbiological tube assay technique was used. Preparation of extracts from the cells and paper-electrophoresis were carried out by means of the same precedures as described previously. The results obtaind showed that at least four kinds of cobamides existed in the cell-extracts. The most active substance was most quickly moving towards cathode and seemed to be Barker's DBCC. Isolation and characterisation of four cobamides above mentioned will be presented in the future.
