Abstract
The medium for the differentiation of Bacillus thiaminaeseus with other relating Bacillus was devised by adding the bromthymol blue to starch nutrient agar. On this medium the acid production is positive for B. thiaminolyticus, B. avei or B. circulans, though is negative for B. thiaminaeseus, B. aneurinolyticus, B. laterosporus or B. brevis. Bacillus thiaminaeseus forms quick spreading greenish blue colony on this medium, while B. thiaminolyticus and other related Bacillus form nonspreading chrome yellow colony, and the both can be distinctly differentiate each other. It is supposed to be recommended for the easy and reliable medium for the isolation of B. thiaminaeseus.
