CHEMICAL STUDIES ON VITAMIN B_<12> AND ITS RELATED COMPOUNDS : (IX) PREPARATION OF 5,6-DIMETHYLBENZIMIDAZOLYLCOBAMIDE COENZYME BY CULTIVATION OF PROPIONIBACTERIUM SHERMANII IN THE MEDIUM CONTAINING HYDROXOCOBALAMIN AND BY PURIFICATION FROM THE COENZYME-RICH CELLS USING SIMPLIFIED PROCEDURE

Abstract

By adding hydroxocobalamin to the culture medium modified by the authors, biosynthesised amounts of DBCC were found to increase strikingly in the cells of Pr. shermanii. From the cells so obtained, crystalline coenzyme was prepared very easily by adopting chromatography on column of P-cellulose as the main purification procedure.