Abstract
In previous papers, the present authors reported that the relationship between vitamin D potency and sulfate metabolism was studied and higher level of sulfate in keel and left tibia of chicks receiving vitamin D_3 was demonstrated using ^<35>S-sulfate. Afterwards, further researches were reported for the action of vitamin D_ in active sulfate metabolism of chicks. The present paper deals with further studies on the microdetermination of vitamin D by paper chromatography. Successful results were obtained for the separation and estimation of vitamin D, provitamin and other sterols using paper chromatography.
