STUDIES ON THE NUTRITIONAL VALUE OF ALLIUM PLANTS : (XL) STUDIES ON THE METHOD OF DETERMINATION OF THIAMINE PROPYLDISULFIDE-^<35>S AND SEVERAL FUNDAMENTAL EXPERIMENTS

Abstract

In order to investigate the physiological properties of thiamine propyldisulfide. TPD-^<35>S was used. This paper deals with studies on the method of determination of TPD-^<35>S and several fundamental experiments. Blood or tissues containing TPD-^<35>S was digested with the mixture of nitric acid and perchloric acid, then ^<35>S was determined as benzidine sulfate-^<35>S. In the case of standard TPD or thiamine solution, ^<35>S was determined directly by thinness method and it was confirmed that the both samples were pure by paper chromatography.