Abstract
Method of separation and determination of mixed tocopherols by gas chromatography using a hydrogen flame ionization detector was investigated. Analysis were carried out on the column packing consisted of Chromosorb W coated with 1.5% SE-30 at a column temperature of 250℃. For the determination of each tocopherol, squalene was used as an internal standard, and the half value width method as a method of measurement of peak areas. After mixed tocopherols were acetylated by the solution of pyridine-acetic anhydride, they were dissolved in acetone, and 5μl of 3% acetone solution of mixed tocopherols was injected into gas chromatography. By this method satisfactory results were obtained with the linearity of calibration curves from 20 to 100 μg of each tocopherol and with the recovery of 96.0 - 103.0%.
