Abstract
Experimental studies were performed on the effect of tryptophan and lysine deficiency in the diet for the biosynthesis of the protein-bound lipoic acid using Nose and Mori's method. Rats were divided into three groups : standard diet, tryptophan deficient- and lysine deficient groups. No remarkable change in the weights of body and liver in all groups was noted. The incorporation rate per mg N in liver mitochondrial protein was highest in the standard diet group, followed by lysine deficient- and tryptophan deficient groups. When lipoic acid-^<35>S was injected the incorporation rate was elevated until first hour in all groups, but the later lowering was faster in lysine- and tryptophan-deficient groups than standard diet group.
