Abstract
In the previous papers, the authors studied the intimate relationship in chicks between vitamin D_3 metabolism and active sulfate metabolism, and activities of vitamin D_3 in active sulfate metabolism was demonstrated using ^<35>S-labeled sulfate. Recently, further investigation was performed for the chemical synthesis of vitamin D_2-sulfate, and its properties were carefully tested. Finally, the sulfate was isolated from the rabbit urine loaded with vitamin D_2.