Abstract
The thiamine content in soybean seedlings increased in vivo by administrating both moieties the pyrimidine and thiazole, and the amount of the vitamin synthesized increased with the concentrations of two moieties supplied. When 2-methyl-4-amino-5-hydroxymethylpyrimidine and thiazole-^<35>S were used, the amount of the vitamin formed which was assayed by the usual thiochrome method was good agreement with that which was calculated from radioactivities of the thiamine-^<35>S synthesized. The vitamin was mainly synthesized in the cotyledon of seedlings in either the light and dark.
