Abstract
Biosynthesis of CoA from 4'-phosphopantetheine was studied, in vitro, and it was confirmed that the latter is almost completely converted to CoA by an enzyme, partially purified from rat liver homogenate, according to the procedure of Hoagland and Novelli, while formation of CoA from pantetheine, pantothenic acid or 4'-phosphopantothenylcysteine was not observed. When liver extract was incubated with addition of the enzyme, the coenzyme value increased about 2 times of that of before incubation, which indicates that precursors of CoA is present in rat liver in almost same level as CoA. The amount of CoA and its precursor in rat liver is observed to run parallel with growth from birth up to 30 days.
