Abstract
Enzymatic method based on sorbyl-CoA formation (Wakil and Hubscher, 1960) was applied to the rat liver extracts for determination of CoA. The stability of CoA and the procedure for extraction of CoA from the rat liver were examined. The following procedure for preparing the liver extract was chosen. Liver homogenate was boiled immediately for 7 minutes, then centrifuged in the cold temperature. The supernatant was adjusted to pH 4.0 and centrifuged after boiling. The supernatant was used for determination of CoA after adjusting pH to 9.0. CoA values obtained by sorbyl-CoA formation were shown to be about a half of those obtained by the sulfanilamide method or of conjugated pantothenic acid values by microbioassay method. These results suggested that there were considerable amounts of bound form of pantothenic acid in the rat liver, besides CoA.
