Abstract
Bioactivities of DBCC were investigated on 2 kinds of experiments ; replicate culture of glia cell-line, established from newborn rat cerebellum and regeneration of sciatic nerve amputated on adult rabbits. The growth curves of glia cell after treatment with DBCC were found to show a more upward than CN- or OH-cobalamin. Accompanying a process of the regeneration of amputated sciatic nerve in adult rabbits, electromyogram pattern drived from M. gastrocnemius become to indicate the recovered nerve function. With daily intravenous injection of DBCC 125μg/kg, the occurances of these patterns were all accerelated and further the amplitude of each patterns was obviously. higher than that of control. RNA value of motor neurons innervated sciatic nerve in spinal cord treated with DBCC was also increased than CN-or OH-cobalamin. 34 patients of neurological disturbances were selected for the clinical application of DBCC. These patients had subjective and/or objective neurological signs, such as neuralgia, paresthesia, hypesthesia, absence of knee jerk, abnormal electromyogram patterns, etc. Most of the cases received 2 or 3 times per week of 25Oμg of DBCC for 11-90 days. By the treatment, an improvement or a disappearance of neurological signs was obtained in 21 of the cases. A marked therapeutic effect of DBCC was observed in a case of toxic neuritis, in that CN- and OH-cobalamin was found ineffective despite massive doses of total 116 mg.
