Abstract
It is an interesting fact that pyridoxal and toxopyrimidine having pyridine ring and pyrimidine ring respectively, were phosphorylated by the same phosphokinase of mouse brain as presented in previous paper. In this report, the substrate specificity of the pyridoxal kinase was studied. It was found that the highly purified enzyme could phosphorylate pyridoxine, pyridoxamine, 4- or 5-deoxypyridoxine, 2-methylmercapto-4-amino-5-hydroxymethylpyrimidine and 2-ethyl-4-amino-5-hydroxymethylpyrimidine, besides pyridoxal and toxopyrimidine, but did not phosphorylate pyrithioxine.
