Abstract
Progress of the formation of riboflavin compounds of glucosyl oligosaccharide from sucrose and riboflavin by intracellular or extracellular enzyme preparations from Leuconostoc mesenteroides giving a high yield of riboflavin compounds of glucosyl oligosaccharide was followed by the paper chromatographic analysis. The synthesis of 5'-D-riboflavin-α-D-glucopyranoside (B_2-glucoside) reached the maximum value in earlier stage of incubation, and thereafter it was decreased, while riboflavin compounds of glucosyl oligosaccharide were produced by receiving glucosyl group successively from sucrose during the decrease of riboflavin glucoside. It was also pointed out that the intracellular enzyme preparation had both riboflavin compounds-producing and decomposing activities.
