Abstract
A microbiological assay of vitamin B_6 with a pulp disk plate technique was performed. A pulp disk plate technique and the tube assay were performed with Saccharomyces carlsbergensis 4228. The values of vitamin B_6 was successfully determined in the liver of rat ingested with a routine diet and a vitamin B_6 deficient diet. A pulp disk plate technique and the tube assay showed similar results. Pulp disk plate method was simpler than tube assay technique but the former was less favorable for the determination with small quantity of material because it was difficult to fix the material on the disk.
