Abstract
Young Wistar rats (male, 15-20 days old ) were injected with 750μc of myoinositol-2^3H per kg body weight, and its incorporation into free myoinositol pools and phospholipid fractions of the liver, kidney and brain was investigated. The rates of incorporation of ^3H in both fractions of these three organs were compared to each other : the highest values were found in the kidney, and the lowest in the brain. The specific activities found in free myoinositol pool and in monophosphoinositide fraction of the liver at 1 hour after the injection were determined to be 1.85×10^5dpm/μmole and 1.45×10^5dpm/μmole, respectively. However, the total amount of the radioactive myoinositol incorporated into the monophosphoinositide was 2.5 times larger than that incorporated into the free myoinositol pool. It is therefore suggested that in the liver, exogeneous myoinositol is not so much diluted by endogeneous myoinositol in phospholipid biosynthesis.
