Abstract
Activities of glutamic-oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in the supernatant and mitochondrial fractions of the liver and kidney of vitamin B_6-deficient rats were investigated. Young male albino rats were used as the material. In a group of the rats, a vitamin B_6-deficient diet was administered for 5-6 weeks. In another group of the rats, an injection of 20mg of penicillamine which is the antagonist of vitamin B_6,was given once a day and repeated on successive two days. In both groups of the rats, the activities of transaminases were markedly decreased in the soluble fraction of liver and kidney cells, but in contrast with soluble fraction not definitely decreased in the mitochondrial fraction. Further, changes in transaminase activities by the addition of pyridoxal phosphate to the cell fractions of B_6 deficient rats were examined. Approximately 20% increase of the activity was observed in the soluble fraction of the liver, but in the mitochondrial fractions no significant elevations were observed.
