Abstract
Thiamine, when preincubated with yeast cells in glucose solution, was almost completely absorbed on cells. The thiamine on cells could not be destroyed on the incubation with crude thiaminase from B.thiaminolyticus or Corbicula, although it was easily released from the cells on the digestion with cell-wall-lytic enzyme from Streptomyces albidoflaous. The stability of protoplasts prepared from thiamine-accumulated Kl.apiculata cells was decreased inversely proportional to thiamine contents. The highly accumulated thiamine on yeast cells was partly released by osmotic shock or during the process of fermentation, corresponding to the decrease of viable cells. The thiamine was completely released from sedimentation fractions of cells, after the treatment with Braun cell-desintegrator. The thiamine accumulated on yeast cells, was found as free thiamine located in the concentrated status between cell-walls and cytoplasmic membranes.
