BIOSYNTHESIS OF THIAMINE : (3) GAS CHROMATOGRAPHY OF PYRIMIDINE MOIETY OF THIAMINE

Abstract

Pyrimidines related to thiamine were detected and analyzed by gas chromatography with hydrogen flame detector. A stainless column, 0.3×150 cm, packed with Neosorb NC (Nishio Kogyo Co.) coated with 15% diethyleneglycol succinate was maintained at 190℃ with a nitorogen flow of 70 ml/min. The retention times of 2-methyl-4-amino-5-aminomethylpyrimidine (I), 2-methyl-4-amino-5-formylpyrimidine (II), 2-methyl-4-amino-5-pyrimidine carboxylic acid (III), and 2-methyl-4-amino-5-hydroxylpyrimidine (IV), trifluoroacetylated in tetrahydrofuran or pyridine were within 10 minutes and the ratio was 1.0,1.0,1.3 and 1.5 respectively. Samples for analysis were prepared about 1 mg/0.1 ml on (I), (II) and (III), and about 2 mg/0.1 ml on (IV).