Abstract
A new enzymatic method for determination of pyridoxal phosphate was studied by using the induced tyrosine transaminase of liver of rats administered with hydrocortisone. The enzyme preparation was obtained from rat liver homogenate with dialysis and heat treatment which rendered it to respond only to pyridoxal phosphate, but neither to pyridoxine phosphate nor to pyridoxamine phosphate. Possible range of determination in the samples was from 10 to 100mμg using the standard curve of authentic pyridoxal phosphate. The enzyme solution can be used for the assay for 2 months, if kept below-20℃. At least 60 estimations of the samples can be performed by the enzyme solution obtained from one rat liver. (Received Jan. 13,1971)
