Abstract
It was shown that purified bacterial thiaminase I catalyzes an exchange reaction of the thiazole moiety of hydroxyalkylthiamine with aniline, pyridine, or cysteine etc., liberating 2-hydroxyalkyl-4-methyl-5-β-hydroxyethylthiazole which was identified by means of paper chromatography and thin layer chromatography. IR Spectrum of purified thiazole moiety liberated from hydroxyethylthiamine was shown to coincide with that of synthetic 2-hydroxyethyl-4-methyl-5-hydoxyethyl-thiazole. (Received Jan. 26,1971)
