Abstract
The successful separation of α, β+γ and δ-tocopherol was obtained by gel filtration through a column of Sephadex LH-20 using chloroform as an eluting solvent. The values of partition coefficient KD of tocopherols and their acetylated products were determined. It was observed that β, γ and δ-tocopherol showed a marked retardation on Sephadex LH-20 due to the presence of hydroxyl group and that the separation of tocopherols were affected by the structures rather than on the molecular size of the substances.
