Abstract
Incorporation of L-dehydroascorbic acid (DAsA) into human erythrocyte proceeded very rapidly, attaining equilibrium within one minute. Appreciable portions of DAsA incorporated into erythrocyte were converted into ascorbate and L-2,3-diketogulonic acid (DKG) in vivo. During the incorporation experiments at 37℃, DAsA in the test solution (isotonic phosphate buffer, pH 7.4) changed rapidly into DKG. The conversion, however, occurred hardly at 10℃. Human erythrocyte uptook DAsA until the intracelluar level reached the same level as that in the test solution. The incorporation rate of DKG into erythrocyte was very low.
