Abstract
During the course of study to prepare a minimum growth medium for Sporolactobacillus inulinus, necessity of minute amount of thiamine and stimulatory effect of p-aminobenzoic acid (PABA) were demonstrated. According to the result of this study a basal minimum medium (BMM) was proposed essential requirement of minute amount thiamines stimulated by PABA in the preceding paper of this series. Curiously, however, it was found that when a natural medium (GYP) was used for preculture instead of the synthetic medium, the tested microorganism could not grow in the BMM. This shows that the BMM is not always satisfactory. Hence, the growth-promoting effects of various amino acids were reexamined. The results showed that cysteine was indispensable and, on the contrary, PABA became unnecessary for growth of this organism. Thus the minimum medium for Sporolactobacillus inulinus was finally determined as follows : glucose, sodium acetate, minerals, biotin, calcium pantothenate, thiamine, valine, leucine, glutamic acid and cysteine.
