Abstract
Availability of commercial preparations of retinyl palmitate (A-pmt) as a practical standard for high-performance liquid chromatographic (HPLC) determination was studied. A-pmt was determined by the following three methods. (1) Vitamin A assay in JP X (JP method). (2) Purified A-pmt was used as a reference standard (HPLC method). (3) A value of absorbance at 326nm obtained by the JP method was corrected by multiplying it with a ratio of A/T (A: peak area of the peak corresponding to A-pmt by HPLC; T: sum of peak area of all the peaks observed on the chromatogram of HPLC). Since the values obtained by the corrected method on the commercial preparations showed good agreement with those by the HPLC method, we concluded that the preparations were useful as practical standards when the corrected method was used to evaluate the concentration of A-pmt.
