Abstract
A new high-perfomance liquid chromatography method and the conventional method of N^1-methylnicotinamide measurement were compared in analysis of urinary N^1-methylnicotinamide excretion in women's students. The N^1-methylnicotinamide value measured by the conventional method was 29.77 ±12.04μmol/daily urine (mean±SD, n=30), and that measured by the new method was 30.55±12.56μmol/daily urine. The correlation coefficient between the values obtained by the two methods was 0.972 (p<0.001), and the regression line was N^1-methylnicotinamide (new method, μmol/daily urine) = 1.015×N^1-methylnicotinamide (conventional method,μmol/daily urine) +0.342. These results show that the N^1-methylnicotinamide values obtained by the new method could be directly comparable with those obtained by the conventional method.
