Abstract
In this study, using high performance capillary electrophoresis (HPCE) with an improved fluorescent detector, we have devoloped the conditions for separation of vitamin B_6 group and proposed a new system for analysis of B_6 group. This method has advantage in mass sensitivity (ca 1 pg- 1 ng) and shortened time of analysis (within 20 min). though B_6 group in fluid including proteins have been difficult to determine, it showed high resolution and reproducibility for B_6 analysis except for PLP in the presence of standard serum proteins, using handmade microdialysis probe.
