Development of a split luciferase biosensor for visualization of VDR ligands in vivo

Abstract

In vivo imaging is analyzing methods to visualize protein-protein interaction and ligand-protein interaction in living cells or animals using fluorescence or luminescence. The split-luciferase technique which is one of the in vivo imaging tools has been used to perform the real-time imaging of many molecules. Vitamin D receptor (VDR) ligands controls the expression of numerous genes via binding to VDR. However, spatiotemporal action of VDR ligands is unclear in living individuals. In the past, we developed the detection tool of VDR ligands using the split-luciferase technique called vitamin D biosensor. In future, vitamin D biosensor is expected as an analyzing tool to elucidate the entirety of vitamin D in vivo.