Basic fibroblast growth factor (bFGF) による遊離骨移植に対する効果

抄録

Bone is the most frequently transplanted tissue in humans. Bone grafting is a common procedure to fill bone defects, such as when a fracture dose not heal properly, or to replace bone lost to tumor surgery or trauma. The grafted bone is resorbed and replaced by new tissue moving from host bone, however, the incorporation through slow remodeling may initially weakens the graft., The purpose of this study was to investigate the effect of basic fibroblast growth factor (bFGF) on the incorporation of a bone graft. bFGF (0, 10, 100, 500, or 1,000ng) was mixed with a collagen gel and implanted into bone grafts taken from rabbit iliums. The bone grafts were put back in the iliums as they were and harvested at 1, 2, and 4 weeks after surgery. X-ray radiographs of the bone grafts were taken using SOFTEX. Histological examination and histomorphometric analysis were performed on bone samples. Treatment with bFGF had greatly stimulated osteogenesis in the bone grafts both radiologically and histologically in comparison with untreated grafts (those filled with plain collagen gel). Of a total of 4 dose-groups, the 100ng group exhibited the best result, but the osteogenesis was poorer in the higherdose groups. These effects were evident as long as 4 weeks postoperatively. These findings suggest that bFGF may be a practical and important candidate for use in bone grafting procedures.