CONFORMATIONAL CHANGE OF RUBELLA VIRUS SPIKE PROTEINS INDUCED BY 2-MERCAPTOETHANOL
1988 年 41 巻 3 号 p. 109-115
詳細
1988 年 41 巻 3 号 p. 109-115
Hemagglutinating (HA) activity of rubella virus was inactivated with 2-mercaptoethanol (2ME) in a dose-dependent manner. But even low concentrations of 2ME, which had little effect on HA activity by themselves, greatly increased the sensitivity of spike polypeptides to the subsequent trypsin treatment. Increased trypsin sensitivity was shown by an enhanced reduction of HA activity and an enhanced proteolytic removal of both E1 and E2 polypeptides from the surface of the virion. The findings indicate that 2ME causes an extensive disruption in the conformation of spikes composed of E1 and E2 polypeptides.
