A NEW SELECTIVE, DIFFERENTIAL AGAR MEDIUM FOR ISOLATION OF VIBRIO CHOLERAE O1: PMT (POLYMYXIN-MANNOSE-TELLURITE) AGAR

Toshio SHIMADA; Riichi SAKAZAKI; Shigeharu FUJIMURA; Kiyoshi NIWANO; Masatoshi MISHINA; Kinjiro TAKIZAWA

doi:10.7883/yoken1952.43.37

A NEW SELECTIVE, DIFFERENTIAL AGAR MEDIUM FOR ISOLATION OF VIBRIO CHOLERAE O1: PMT (POLYMYXIN-MANNOSE-TELLURITE) AGAR

Toshio SHIMADA, Riichi SAKAZAKI, Shigeharu FUJIMURA, Kiyoshi NIWANO, Masatoshi MISHINA, Kinjiro TAKIZAWA

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JOURNAL FREE ACCESS

1990 Volume 43 Issue 2 Pages 37-41

DOI https://doi.org/10.7883/yoken1952.43.37

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Abstract

A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (TCBS) agar.

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