Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
Volume 43, Issue 2
Displaying 1-4 of 4 articles from this issue
  • Sumalee BOONMAR, Boonsong POJANAGAROON, Yushiro WATANABE, Yukie TANAKA ...
    1990 Volume 43 Issue 2 Pages 29-36
    Published: 1990
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    With the antigen expressed in yeast from a cDNA clone encoding a non-structural region of newly discovered hepatitic C virus (HCV) genome, the prevalence of HCV antibody in people in Thailand was investigated. Antibody was detected in 2.6% of healthy blood donors and in 2.8% of healthy pregnant women. These prevalence rates were higher than those reported previously from Japan, USA and European countries. Among community-acquired, sporadic cases of acute and chronic non-A, non-B hepatitis, however, only 5.7% and 15.4% were shown to possess the antibody, respectively. Among hepatocellular carcinoma patients who were negative for hepatitis B surface antigen in the sera, 11.1% had antibody to HCV. These seroepidemiological data suggest that HCV plays an important role as an etiological agent in Thailand; however, other agents must also be involved in etiologic agents of viral hepatitis and chronic liver disease.
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  • Toshio SHIMADA, Riichi SAKAZAKI, Shigeharu FUJIMURA, Kiyoshi NIWANO, M ...
    1990 Volume 43 Issue 2 Pages 37-41
    Published: 1990
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (TCBS) agar.
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  • Koji FURUYA, Mariko NISHIZUKA, Hiroshi HONMA, Mitsuru KUMAGAI, Naoki S ...
    1990 Volume 43 Issue 2 Pages 43-49
    Published: 1990
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    The Western blotting (WB) method was used to evaluate the prevalence of human alveolar echinococcosis in Hokkaido, Japan. One hundred and sixteen WB-positive serum specimens were found in 1987 and 1988, and were grouped into two serologically different types from their WB-immunostaining patterns: “complete type”, that showed multiple bands with various molecular weights ranging from 29 to 205 kilodaltons (kDa), and “incomplete type”, that revealed a few bands with low molecular weights of 30-35 kDa and/or with molecular weights higher than 90 kDa. Therefore, we found that the WB method is applicable for the sero-epidemiological studies of human alveolar echinococcosis in Hokkaido.
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  • Kikuko MIYAMURA, Kazuyo YAMASHITA, Shizuko YAMADERA, Nobuko KATO, Masa ...
    1990 Volume 43 Issue 2 Pages 51-58
    Published: 1990
    Released on J-STAGE: March 19, 2010
    JOURNAL FREE ACCESS
    Laboratory reports of isolation of echovirus serotype 18 (E18) slightly increased in the summer of 1987 followed by a sharp increase with a peak in July, 1988. A total of 1, 094 isolations were reported during these two years from 39 laboratories participating in the National Epidemiological Surveillance of Infectious Agents in Japan. When compared with the previous E18 outbreaks, a higher proportion of children at two years of age or under (58.3%) and a much higher incidence of exanthem (46.4%) were remarkable. Meningitis-associated isolations were reported in 30.6%, less than half of the percentage of the previous epidemic.
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