抄録
As reported in the first report of the present series, α-glucosidase of Aerobacter cloacae etc participates in the fermentation of sucrose. In the absence of α-glucosidase, however, if fructosidase is present, sucrose will be hydrolyzed into fructose and glucose. Emil Fischer et al proved the above facts by using yeast and such fermentation is called as indirect fermentation. While, it has been insisted that the disaccharide such as sucrose or maltose can be fermented without the process of hydrolysis and numerous studies based on different standpoints have been reported on this problem.
Regarding the sucrose fermentation of living A. cloacae and Esch. coli var. communior etc., as reported before, the oxygen bridge at the α-glucoside conjunction of sucrose is hydrolyzed by α-glucosidase in the former, while, in the latter, as it has no α-glucosidase, the fermentation either by fructosidase or of some other type must take place. It is considered, therefore, that the enzyme participating in the fermentation of sucrose is entirely different between A. cloacae and Esch. coli var. communior. Experiments were carried out in order to know the difference of sucrose fermentation between those two strains, and some results, which indicated the presence of direct fermentation in the sucrose fermentation of Esch. coli communior, have been obtained.
