総説および総合論文
疎水性精製タグを導入した新規キャップアナログの合成と完全キャップ化メッセンジャーRNA製造への応用
2025 年 83 巻 1 号 p. 2-14
詳細
2025 年 83 巻 1 号 p. 2-14
Messenger RNA (mRNA) is gaining attention as a new modality of oligonucleotide therapeutics. We have reported a new mRNA synthesis method, named the PureCap method, by in vitro transcription, which enables the production of completely capped mRNA. For this purpose, we designed and synthesized new cap analogs bearing hydrophobic o-nitrobenzyl groups, which function as purification tags for mRNA with a 5′-cap structure by reverse-phase high-performance liquid chromatography. In this paper, we describe the synthesis of new cap analogs, named PureCap analogs, and its application for completely capped mRNA synthesis.
