An earthquake, whose magnitude was 4.9, occurred in the southern part of Lake Biwa on January 11, 1990. The epicenter of it was just near Moriyama observation well, where we continuously measured many geochemical and hydrological parameters for earthquake prediction research from April, 1983. These data were carefully compared with the water level data of Lake Biwa and the meteorological factors to check any possible relationship. We concluded that nothing but the groundwater temperature at a depth of 780m anomalously changed before the earthquake.